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Invitrogen
Nesprin 1 Polyclonal Antibody
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Application
FIGURE: 1 / 14
Nesprin 1 Antibody (PA5-115640) in ICC/IF
Immunocytochemistry analysis of Nesprin 1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115640) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat a... View More
Product Details
PA5-115640
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SYNE1(Accession Q8NF91), corresponding to amino acid residues S8670-T8720.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900275
Product Specific Information
Antibody detects endogenous levels of total Nesprin 1.
Target Information
There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-l and -2 for nuclear _envelope _spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as 'dystrophins of the nucleus' to maintain nuclear organization and structura integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CPG2 full length; DKFZp781J13156; Enaptin; enaptin165; FLJ30878; FLJ41140; KASH domain-containing protein 1; KASH1; Myne-1; Myocyte nuclear envelope protein 1; nesprin 1; Nesprin-1; Nuclear envelope spectrin repeat protein 1; RP1-130E4.2; spectrin repeat containing, nuclear envelope 1; synaptic nuclear envelope 1; Synaptic nuclear envelope protein 1; synaptic nuclei expressed gene 1; SYNE 1; Syne-1; syne-1 fragment
Gene Aliases: 8B; A330049M09Rik; ARCA1; BE692247; C130039F11Rik; C6orf98; CPG2; dJ45H2.2; EDMD4; KIAA0796; KIAA1262; KIAA1756; mKIAA1756; Myne-1; MYNE1; Nesp1; SCAR8; SYNE1
UniProt ID: (Human) H0Y4C0, (Mouse) Q6ZWR6
Entrez Gene ID: (Human) 23345, (Mouse) 64009, (Rat) 499010
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