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NeuroD1 Antibody (MA5-55297) in WB
Western blot analysis of lysates from mouse brain, using NeuroD1 Recombinant Monoclonal Antibody (Product # MA5-55297) at 1:1,000 dilution followed by an HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-55297
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5V4L8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NeuroD1.
Conjugate
Form
Liquid
Concentration
1.33 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DDLETMNAEE DSLRNGGEEE DEDEDLEEEE EEEEEDDDQK PKRRGPKKKK MTKARLERFK
Target Information
NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-cell E-box transcriptional activator 2; Beta2; BHF1; neuro d1; NeuroD1; Neurogenic differentiation factor 1
Gene Aliases: BETA2; BHF-1; bHLHa3; Neurod; Neurod1
UniProt ID: (Mouse) Q60867
Entrez Gene ID: (Mouse) 18012
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
E-box binding
basic helix-loop-helix transcription factor
pancreatic A cell fate commitment
pancreatic PP cell fate commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
nucleocytoplasmic transport
nitric oxide mediated signal transduction
multicellular organism development
nervous system development
response to glucose
anterior/posterior pattern specification
dentate gyrus development
cerebellum development
signal transduction involved in regulation of gene expression
insulin secretion
cell differentiation
hindbrain development
endocrine pancreas development
amacrine cell differentiation
enteroendocrine cell differentiation
glucose homeostasis
camera-type eye development
positive regulation of apoptotic process
cell fate commitment
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
embryonic organ morphogenesis
inner ear development
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of intestinal epithelial structure maintenance
regulation of cell cycle arrest
cellular response to glucose stimulus
negative regulation of type B pancreatic cell apoptotic process
positive regulation of transcription regulatory region DNA binding
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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