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Invitrogen
Neutrophil elastase Polyclonal Antibody
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Application
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Neutrophil elastase Antibody (PA5-79198) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Neutrophil elastase in paraffin-embedded section of mouse spleen tissue using Neutrophil elastase Polyclonal Antibody (Product # PA5-79198). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/... View More
Product Details
PA5-79198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746314
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse spleen tissue. IHC: mouse spleen tissue, rat spleen tissue.
Target Information
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; Leukocyte elastase; Neutrophil elastase; PE-1
Gene Aliases: Ela2; Elane; F430011M15Rik; NE
UniProt ID: (Mouse) Q3UP87
Entrez Gene ID: (Rat) 299606, (Mouse) 50701
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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