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Nicastrin Antibody (A304-464A-T) in WB
Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody A304-464A (lot A304-464A-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated Nicastrin, A304-464A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-464A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 659 to 709 of human Nicastrin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840
Gene Aliases: ATAG1874; KIAA0253; NCSTN; UNQ1874/PRO4317
UniProt ID: (Human) Q92542
Entrez Gene ID: (Human) 23385
myeloid cell homeostasis
proteolysis
membrane protein ectodomain proteolysis
Notch signaling pathway
Notch receptor processing
protein processing
membrane protein intracellular domain proteolysis
T cell proliferation
amyloid precursor protein catabolic process
positive regulation of apoptotic process
positive regulation of catalytic activity
ephrin receptor signaling pathway
beta-amyloid metabolic process
epithelial cell proliferation
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