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Invitrogen
Nicastrin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Nicastrin Antibody (PA5-17735) in WB
Western blot was performed using Nicastrin Polyclonal Antibody (Product # PA5-17735) and a 130 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of T98G (Lane 1), RSC96 (Lane 2), U-87 MG (Lane 3), IMR32 (Lane 4), Neuro 2a (Lane 5), HeLa (Lane 6), Hep G2 (Lane 7), MCF7 (Lane 8), Mouse Brain (Lane 9), Mouse Heart (Lane 10) and Rat Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then t... View More
Product Details
PA5-17735
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the carboxy-terminus of human Nicastrin
Conjugate
Form
Liquid
Concentration
183 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981453
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840
Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317
UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6
Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231
myeloid cell homeostasis
proteolysis
membrane protein ectodomain proteolysis
Notch signaling pathway
protein processing
T cell proliferation
positive regulation of catalytic activity
beta-amyloid metabolic process
epithelial cell proliferation
Notch receptor processing
membrane protein intracellular domain proteolysis
amyloid precursor protein catabolic process
positive regulation of apoptotic process
ephrin receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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