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Alomone Labs, LTD
Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Polyclonal Antibody
Application
FIGURE: 1 / 2
Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Antibody (ANC-013-200UL) in ICC/IF
Expression of nAChR beta 3 in live intact rat PC12 cells - Cell surface detection of nAChR beta 3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge image of A and B.
Product Details
ANC-013-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChR beta 3, Extracellular, N-terminus
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
The nicotinic acetylcholine receptors are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; cholinergic receptor; cholinergic receptor, nicotinic, beta polypeptide 3; Neuronal acetylcholine receptor subunit beta-3; neuronal nicotinic
Gene Aliases: 5730417K16Rik; Acrb3; Chrnb3
UniProt ID: (Rat) P12391, (Mouse) Q8BMN3
Entrez Gene ID: (Rat) 171131, (Mouse) 108043
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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