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Invitrogen

Nkx3.1 Monoclonal Antibody (3 9)

12 Published Figures

6 References

View all (40) Nkx3.1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Catalog # 35-9700

Price not available

100 µg

Contáctenos

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (12)

Application

Nkx3.1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

FIGURE: 1 / 12

Nkx3.1 Antibody (35-9700) in WB, ICC/IF

F1000Research 2016 - Figure S1. Transfection of FLAG-NKX3.1 expression into LNCaP cells and affinity purification. ( A ) LNCaP cells were transfected with pFLAG-NKX3.1 plasmid or with the empty pFLAG vector. Total cell lysate (lanes 1 and 2) was absorbed to anti-FLAG resin and eluted with FLAG peptides (lanes 5 and 6). The depleted cell lysate after affinity purification is shown in lanes 3 and 4. Immunoblots were probed with t... View More

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Product Details

35-9700

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3 9

Immunogen

Fusion protein containing the N-terminal domain of the human Nkx3.1 protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533232

Target Information

The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A three-protein biomarker panel assessed in diagnostic tissue predicts death from prostate cancer for men with localized disease.

Cancer medicine

Severi G,FitzGerald LM,Muller DC,Pedersen J,Longano A,Southey MC,Hopper JL,English DR,Giles GG,Mills J

35-9700 was used in immunohistochemistry to investigate the prognostic significance of a three-protein biomarker panel for prostate cancer

Wed Oct 01 00:00:00 UTC 2014

Systems analysis of the prostate tumor suppressor NKX3.1 supports roles in DNA repair and luminal cell differentiation.

F1000Research

Yang CC,Chung A,Ku CY,Brill LM,Williams R,Wolf DA

35-9700 was used in western blot to study the roles of prostate tumor suppressor NKX3.1 in DNA repair and luminal cell differentiation

Fri Sep 16 00:00:00 UTC 2016

Systems analysis of the prostate tumor suppressor NKX3.1 supports roles in DNA repair and luminal cell differentiation.

F1000Research

Yang CC,Chung A,Ku CY,Brill LM,Williams R,Wolf DA

35-9700 was used in western blot to study the roles of prostate tumor suppressor NKX3.1 in DNA repair and luminal cell differentiation

Fri Sep 16 00:00:00 UTC 2016

TWIST1, A novel androgen-regulated gene, is a target for NKX3-1 in prostate cancer cells.

Cancer cell international

Eide T,Ramberg H,Glackin C,Tindall D,Taskén KA

35-9700 was used in chip-sequencing to elucidate the metastatic process.

Thu Jan 31 00:00:00 UTC 2013

NKX3.1 homeodomain protein binds to topoisomerase I and enhances its activity.

Cancer research

Bowen C,Stuart A,Ju JH,Tuan J,Blonder J,Conrads TP,Veenstra TD,Gelmann EP

Mon Jan 15 00:00:00 UTC 2007

NKX3.1 homeodomain protein binds to topoisomerase I and enhances its activity.

Cancer research

Bowen C,Stuart A,Ju JH,Tuan J,Blonder J,Conrads TP,Veenstra TD,Gelmann EP

Mon Jan 15 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Drosophila NK-3 transcription factor, locus 1; Homeobox protein NK-3 homolog A; Homeobox protein Nkx-3.1; NK homeobox, family 3, A; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1; NKX3-1 antibody; prostate-specific ag

Gene Aliases: bagpipe; BAPX2; Bax; Nkx-3.1; NKX3; NKX3-1; NKX3.1; NKX3A

UniProt ID: (Human) Q99801, (Mouse) P97436

Entrez Gene ID: (Human) 4824, (Mouse) 18095

Function(s)

transcription regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter binding transcription factor activity, sequence-specific DNA binding androgen receptor activity protein binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process estrogen receptor activity protein kinase activator activity estrogen receptor binding histone deacetylase binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding MADS box domain binding DNA binding homeodomain transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nkx3.1 Monoclonal Antibody (3 9)

Nkx3.1 Antibody (35-9700) in WB, ICC/IF

Product Details

35-9700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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