Loading ...

Invitrogen

Nkx6.1 Polyclonal Antibody

View all (11) Nkx6.1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

FIGURE: 1 / 1

Nkx6.1 Antibody (PA5-103899) in WB

Western blot analysis of Nkx6.1 in HepG2 cell lysate. Samples were incubated with Nkx6.1 polyclonal antibody (Product # PA5-103899).

Product Image Gallery

Product Details

PA5-103899

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NKX6-1(Accession P78426), corresponding to amino acid residues M209-K259.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2853231

Product Specific Information

Antibody detects endogenous levels of total NKX61.

Target Information

NKX6.1 is a bi-functional transcription factor that has effects in both the central nervous system and in the pancreas. It has both expression and repression effects on genes, leading to the proper formation of motoneurons, oligodendrocytes, and insulin-secreting beta cells. It is known to interact with Pax6 and Pdx-1, probably at or near the 60 amino acid homeobox DNA-binding domain. Due to its obvious relationship to beta cells and CNS, NKX6.1 is becoming a more interesting target for therapies in diabetes and certain brain diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Drosophila NK transcription factor related, gene family 6, locus 1; Homeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; homeodomain transcription factor Nkx6.1; NK homeo box, family 6, member A; NK homeobox, family 6, A; NK6 transcription factor homolog A; NK6 transcription factor related locus 1; NK6 transcription factor related, locus 1

Gene Aliases: NKX6-1; NKX6.1; Nkx61; NKX6A

UniProt ID: (Human) P78426, (Mouse) Q99MA9, (Rat) O35762

Entrez Gene ID: (Human) 4825, (Mouse) 18096, (Rat) 65193

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding homeodomain transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter neuron migration transcription from RNA polymerase II promoter smoothened signaling pathway organ morphogenesis regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of axon extension pancreas development positive regulation of insulin secretion response to nicotine response to drug type B pancreatic cell proliferation positive regulation of neuron differentiation negative regulation of glial cell differentiation positive regulation of glial cell differentiation positive regulation of transcription from RNA polymerase II promoter oligodendrocyte differentiation positive regulation of sequence-specific DNA binding transcription factor activity detection of glucose cellular response to cytokine stimulus cellular response to peptide hormone stimulus type B pancreatic cell maturation positive regulation of type B pancreatic cell development regulation of neuron migration regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development cell proliferation central nervous system neuron differentiation neurogenesis cell differentiation endocrine pancreas development type B pancreatic cell development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more