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Nogo-A Antibody (PA5-47498) in ICC/IF
Immunocytochemistry analysis of Nogo-A in immersion fixed 7 day differentiated rat cortical stem cells. Samples were incubated in Nogo-A polyclonal antibody (Product # PA5-47498) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Product Details
PA5-47498
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human Nogo-A Val566-Phe748
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2577078
Product Specific Information
In direct ELISAs, approximately 5% cross-reactivity with recombinant rat Nogo-A (aa 543-725) and recombinant human Nogo-B is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Foocen; Human NogoA; My043 protein; neurite growth inhibitor 220; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; Nogo protein; NSP; reticulon 5; Reticulon-4; Reticulon-5; RTN-x
Gene Aliases: ASY; KIAA0886; My043; Nbla00271; Nbla10545; NI220/250; NOGO; NOGO-A; Nogo-B; Nogo-C; NOGOC; NSP; NSP-CL; RTN-X; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; SP1507
UniProt ID: (Human) Q9NQC3
Entrez Gene ID: (Human) 57142
apoptotic process
axonal fasciculation
cerebral cortex radial glia guided migration
negative regulation of cell growth
negative regulation of axon extension
regulation of apoptotic process
negative regulation of axonogenesis
nuclear pore complex assembly
cardiac epithelial to mesenchymal transition
endoplasmic reticulum tubular network organization
endoplasmic reticulum tubular network assembly
cell-cell adhesion
regulation of branching morphogenesis of a nerve
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