OriGene

Notch1 Monoclonal Antibody (OTI3E12), TrueMAB™

2 References
View all (50) NOTCH1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

TA500078

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:50
-

Miscellaneous PubMed (Misc)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI3E12

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Neurogenic locus notch homolog protein 1; Notch 1; Notch homolog 1, translocation-associated; RP23-306D20.12; RP23-306D20.12-001; Translocation-associated notch protein TAN-1

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Gene Aliases: AOS5; AOVD1; hN1; NOTCH1; TAN1

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UniProt ID: (Human) P46531

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Entrez Gene ID: (Human) 4851

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Function(s)
core promoter binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding enzyme inhibitor activity receptor activity Notch binding calcium ion binding protein binding enzyme binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity cell adhesion molecule
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate specification epithelial to mesenchymal transition liver development heart looping sprouting angiogenesis positive regulation of neuroblast proliferation inflammatory response to antigenic stimulus endocardium development endocardium morphogenesis atrioventricular node development coronary vein morphogenesis aortic valve morphogenesis atrioventricular valve morphogenesis pulmonary valve morphogenesis mitral valve formation epithelial to mesenchymal transition involved in endocardial cushion formation endocardial cushion morphogenesis cardiac chamber formation cardiac ventricle morphogenesis cardiac atrium morphogenesis cardiac right atrium morphogenesis cardiac left ventricle morphogenesis cardiac right ventricle formation ventricular trabecula myocardium morphogenesis growth involved in heart morphogenesis regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Notch signaling pathway involved in regulation of secondary heart field cardioblast proliferation cell migration involved in endocardial cushion formation pericardium morphogenesis regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter immune response humoral immune response Notch signaling pathway positive regulation of transcription of Notch receptor target spermatogenesis determination of left/right symmetry compartment pattern specification axonogenesis foregut morphogenesis endoderm development heart development positive regulation of cell proliferation negative regulation of cell proliferation auditory receptor cell fate commitment positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion negative regulation of myotube differentiation mesenchymal cell development regulation of somitogenesis cell differentiation in spinal cord neural tube development keratinocyte differentiation negative regulation of ossification lung development positive regulation of cell migration positive regulation of BMP signaling pathway negative regulation of BMP signaling pathway forebrain development hair follicle morphogenesis organ regeneration response to corticosteroid response to muramyl dipeptide response to lipopolysaccharide embryonic hindlimb morphogenesis tube formation skeletal muscle cell differentiation cellular response to vascular endothelial growth factor stimulus tissue regeneration positive regulation of apoptotic process negative regulation of catalytic activity positive regulation of viral genome replication positive regulation of endothelial cell differentiation negative regulation of auditory receptor cell differentiation positive regulation of keratinocyte differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis positive regulation of JAK-STAT cascade negative regulation of photoreceptor cell differentiation somatic stem cell division astrocyte differentiation oligodendrocyte differentiation positive regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation branching morphogenesis of an epithelial tube positive regulation of viral transcription positive regulation of epithelial cell proliferation negative regulation of neurogenesis cardiac muscle tissue morphogenesis cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation negative regulation of glial cell proliferation cilium morphogenesis cardiac epithelial to mesenchymal transition cardiac septum morphogenesis ventricular septum morphogenesis secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development prostate gland epithelium morphogenesis regulation of epithelial cell proliferation involved in prostate gland development arterial endothelial cell differentiation venous endothelial cell differentiation cardiac vascular smooth muscle cell development endocardial cell differentiation vasculogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis Notch signaling involved in heart development heart trabecula morphogenesis positive regulation of transcription from RNA polymerase II promoter in response to hypoxia left/right axis specification cellular response to follicle-stimulating hormone stimulus distal tubule development collecting duct development glomerular mesangial cell development interleukin-4 secretion negative regulation of cell migration involved in sprouting angiogenesis negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance regulation of extracellular matrix assembly apoptotic process involved in embryonic digit morphogenesis negative regulation of stem cell differentiation negative regulation of anoikis negative regulation of pro-B cell differentiation negative regulation of endothelial cell chemotaxis
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