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FIGURE: 1 / 8
OCT1 (POU2F1) Antibody (PA5-28209) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of OCT1 (POU2F1) was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 (POU2F1) Polyclonal Antibody (Product # PA5 28209) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28209
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 442 and 755 of Human OCT1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545685
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2.
Predicted reactivity: Mouse (89%), Rat (94%), Xenopus laevis (81%), Pig (97%), Chicken (94%), Chimpanzee (99%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NF-A1; Oct-1; Octamer-binding protein 1; Octamer-binding transcription factor 1; Octamer-binding transcription factor-1; OTF-1; POU domain, class 2, transcription factor 1
Gene Aliases: Oct-1; oct-1B; OCT1; Otf-1; OTF1; POU2F1
UniProt ID: (Human) P14859, (Rat) P31503
Entrez Gene ID: (Human) 5451, (Rat) 171068
Performance Guarantee
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