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FIGURE: 1 / 1
OTC Antibody (PA5-97260) in WB
Western blot analysis of OTC in extracts of various cell lines. Samples were incubated with OTC Polyclonal antibody (Product # PA5-97260) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-97260
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_0005223)
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809062
Product Specific Information
Immunogen sequence: LQAATPKGYE PDASVTKLAE QYAKENGTKL LLTNDPLEAA HGGNVLITDT WISMGQEEEK KKRLQAFQGY QVTMKTAKVA ASDWTFLHCL PRKPEEVDDE VFYSPRSLVF PEAENRKWTI MAVMVSLLTD YSPQLQKPKF; Positive Samples: Mouse liver, Mouse small intestine, Rat liver, Rat small intestine; Cellular Location: Mitochondrion matrix
Target Information
This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ornithine carbamoyltransferase, mitochondrial; ornithine transcarbamylase; Ornithine transcarbamylase, mitochondrial; OTCase; sparse fur
Gene Aliases: AI265390; OCTD; OTC; Sf; spf
UniProt ID: (Human) P00480, (Mouse) P11725, (Rat) P00481
Entrez Gene ID: (Human) 5009, (Mouse) 18416, (Rat) 25611
urea cycle
liver development
ornithine catabolic process
midgut development
response to zinc ion
citrulline biosynthetic process
response to insulin
arginine biosynthetic process via ornithine
response to drug
anion homeostasis
protein homotrimerization
response to biotin
ammonia homeostasis
cellular amino acid metabolic process
arginine biosynthetic process
ornithine metabolic process
cellular amino acid biosynthetic process
response to nutrient levels
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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