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            • Primary Antibodies ›
            • SIINFEKL Antibodies

            Invitrogen

            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE, eBioscience™

            15 Published Figures
            36 References
            View all (11) SIINFEKL antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 12-5743-82
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            Cite OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (15)
            Application
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 16

            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody (12-5743-82) in Flow

            Staining of BALB/c (left) or C57BL/6 splenocytes (right) either unpulsed (open histogram) or pulsed with OVA257-264 (SIINFEKL) peptide (filled histogram) with 0.25 µg of Anti-Mouse OVA257-264 (SIINFEKL) peptide bound to H-2Kb PE. Total viable cells were used for analysis.
            View Product

            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)
            OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)

            Product Details

            12-5743-82

            Applications
            Tested Dilution
            Publications

            Immunocytochemistry (ICC/IF)

            -
            View 2 publications 2 publications

            Flow Cytometry (Flow)

            0.5 µg/test
            View 33 publications 33 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Human, Mouse, Yeast

            Host/Isotype

            Mouse / IgG1, kappa

            Recommended Isotype Control

            Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            eBio25-D1.16 (25-D1.16)

            Conjugate

            PE
            • Unconjugated
            • APC 
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 615
            • Brilliant UV 737
            • Brilliant Violet 650
            • Brilliant Violet 711
            • PE-Cyanine7
            • PerCP-eFluor 710
            • Request custom conjugation

            Excitation/Emission Max

            565/576 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_925774

            Product Specific Information

            Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

            Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Cells can be pulsed with the SIINFEKL peptide according to the following protocol:

            1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.

            2. Incubate cells at 37°C for 2 hours.

            3. Wash cells with flow staining buffer.

            4. Proceed with cell surface staining as normal.

            For additional information see the references listed below.

            Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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