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Invitrogen
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, eBioscience™
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20240507 "OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 11
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody (13-5743-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (left) or 0.06 µg of Anti-Mouse OVA (257-264) (SIINFEKL) peptide bound to H-2Kb Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20240507)
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20240507?time=20240507 "OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody (13-5743-82) in Flow")
Product Details
13-5743-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio25-D1.16 (25-D1.16)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1210600
Product Specific Information
Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.
Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.
Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Cells can be pulsed with the SIINFEKL peptide according to the following protocol:
1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.
2. Incubate cells at 37°C for 2 hours.
3. Wash cells with flow staining buffer.
4. Proceed with cell surface staining as normal.
For additional information see the references listed below.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-2Kb-SIINFEKL; OVA-Kb; OVA257-264; OVA257-264 H-2Kb
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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