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OXPAT Antibody (PA1-46216) in WB
Western blot analysis of OXPAT in 0.5 mg/mL Human Heart (left) and Mouse Heart (right) lysate. Samples were incubated in OXPAT polyclonal antibody (Product # PA1-46216). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Product Details
PA1-46216
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
C-terminal region of the mouse OXPAT protein, with in residues 400-463 [Uniprot# Q8BVZ1].
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_1017919
Product Specific Information
The target sequence has 89% sequence homology with rat and 75% sequence homology with bovine.^M
^M
Suggested positive control: human and mouse heart lysate.
Target Information
Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lipid droplet associated protein; Lipid droplet-associated protein PAT-1; Lipid storage droplet protein 5; Myocardial LD protein; Perilipin-5
Gene Aliases: 2310076L09Rik; AI415325; AW109675; LSDA5; LSDP5; MLDP; OXPAT; PAT-1; Pat1; PLIN5
UniProt ID: (Human) Q00G26, (Mouse) Q8BVZ1
Entrez Gene ID: (Human) 440503, (Mouse) 66968
positive regulation of triglyceride biosynthetic process
positive regulation of lipid storage
positive regulation of sequestering of triglyceride
negative regulation of triglyceride catabolic process
lipid storage
negative regulation of fatty acid beta-oxidation
positive regulation of fatty acid beta-oxidation
lipid particle organization
negative regulation of peroxisome proliferator activated receptor signaling pathway
mitochondrion localization
negative regulation of lipase activity
positive regulation of lipase activity
negative regulation of reactive oxygen species metabolic process
lipid metabolic process
negative regulation of lipid catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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