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Invitrogen
Oligodendrocyte Marker O1 Monoclonal Antibody (O1), eBioscience™
Application
FIGURE: 1 / 1
Oligodendrocyte Marker O1 Antibody (14-6506-82) in ICC/IF
Immunocytochemistry of fixed, differentiated OLN93 cells using 10 µg/mL of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (left) or 10 µg/mL Anti-Oligodendrocyte Marker O1 (right) followed by Anti-Mouse IgM FITC (Product # 11-5790-81). Nuclei are stained with DAPI.
Product Details
14-6506-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
O1
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10718397
Product Specific Information
Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors. O1 has been reported to react to all species tested (rat, mouse, human, chicken). Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, ELISA, and immunocytochemistry.
Applications Tested: This O1 antibody has been tested by immunocytochemistry on formaldehyde-fixed, differentiated OLN93 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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