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Invitrogen
Ovalbumin Polyclonal Antibody, HRP
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Application
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Ovalbumin Antibody (PA1-196-HRP) in WB
Western blot analysis of ovalbumin was performed by loading the indicated amounts of ovalbumin protein (Product # 77120), and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for at least 1 hour at room temperature. A doublet of Ovalbumin protein was detected between 40-50 kD using an HRP-conjugated ovalbumin polyclonal antibody (P... View More
Product Details
PA1-196-HRP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Ovalbumin
Conjugate
HRP
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with proprietary stabilizer
Contains
0.07% Kathon
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539922
Product Specific Information
By Western blot, PA1-196-HRP detects ovalbumin at ~45kD. Multiple bands may be observed, which are likely due to the presence of multiple glycosylated and/or aggregated species of ovalbumin within a particular protein preparation.
Target Information
Chicken ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoi and lysozyme. It belonds to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Allergen Gal d II; clade (ovalbumin); Egg albumin; member 14; Ovalbumin; Plakalbumin; serpin peptidase inhibitor; unnamed protein product
Gene Aliases: OVA; SERPINB14
UniProt ID: (Chicken) P01012
Entrez Gene ID: (Chicken) 396058
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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