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            • Primary Antibodies ›
            • OxPhos Rodent WB Antibodies

            Invitrogen

            OxPhos Rodent WB Antibody Cocktail

            20 Published Figures
            54 References

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            Datasheet
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            Questions & Answers
            Catalog # 45-8099
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            300 µg
             
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            Cite OxPhos Rodent WB Antibody Cocktail

            • Antibody Testing Data (1)
            • Published Figures (20)
            Application
            OxPhos Rodent WB Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 21

            OxPhos Rodent WB Antibody (45-8099) in WB

            Western blot analysis of OxPhos in Rat liver mitochondria using an OxPhos Monoclonal antibody cocktail (Product # 45-8099). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.
            View Product

            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)
            OxPhos Rodent WB Antibody in Western Blot (WB)

            Product Details

            45-8099

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            Assay-dependent
            View 54 publications 54 publications
            Product Specifications

            Species Reactivity

            Bovine, Human, Mouse, Non-human primate, Rat

            Published species

            Human, Mouse, Rat, Sheep

            Host/Isotype

            Mouse / Ig

            Class

            Cocktail

            Type

            Antibody

            Clone

            Cocktail

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1.5 mg/mL

            Purification

            purified

            Storage buffer

            HEPES buffered saline

            Contains

            0.02% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2533835

            Product Specific Information

            This product is a cocktail of multiple OxPhos antibodies.

            C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
            Purification Method: Antigen Affinity Chromatography

            C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
            Purification Method: IgG Fraction

            C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
            Purification Method: IgG Fraction

            C-IV-I (clone) 1D6E1A8 - Human Complex IV subunit I
            Purification Method: Ammonium Sulfate Precipitation

            C-V-alpha (clone) 15H4C4 - Bovine Complex V
            Purification Method: Antigen Affinity Chromatography

            The kit is suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from mouse, rat, human, or bovine sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50ºC before loaded on the gel.

            The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting. We recommend using PBS with 1% milk as the antibody diluent and using a high pH CAPS/PVDF transfer protocol.

            Target Information

            Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: 13G12AF12BB11; 15H4C4; 1D6E1A8; 20E9DH10C12; 21A11AE7; OxPhos Complex

            View more View less

            It has to be done as per old AB suggested Products section.
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