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MUbio

P53 (TP53 tumor suppressor protein) Monoclonal Antibody (DO-1)

Name: P53 (TP53 tumor suppressor protein) Monoclonal Antibody (DO-1)
Brand: MUbio
SKU: MUB1501P
Price: 444.0 USD

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Catalog # MUB1501P

Price (USD)

444.00

100 µg

Estimated availability date: 23-Jun-2025

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Datasheet

Protocols

Questions & Answers

Product Details

MUB1501P

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

1:500-1:1,000

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

DO-1

Immunogen

P53 (TP53 tumor suppressor protein)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

DO-1 recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (amino acids 11-25) of p53.

The DO-1 antibody is suitable for the detection of p53 by Western blotting and detects a band of 53kDa in tumor tissue and cell extracts.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

Gene Aliases: BCC7; LFS1; P53; TP53; TRP53

UniProt ID: (Human) P04637

Entrez Gene ID: (Human) 7157

Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding P53-like transcription factor

Process(es)

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