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Application
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FOXA3 Antibody (PA1-813) in WB
Western blot analysis of FOXA3 was performed by loading 25 µg of HepG2 (lane 1) mouse liver (lane 2) and rat liver (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a FOXA3 polyclonal antibody (Product # PA1-813) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotti... View More
Product Details
PA1-813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2105002
Product Specific Information
PA1-813 detects FOXA3 from human samples.
PA1-813 has been successfully used in Western blot and ChIP applications. By Western blot, PA1-813 detects a ~37 kDa band representing FOXA3 from mouse liver.
The PA1-813 immunogen is a synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3. This immunizing peptide (PEP-299) is available for use in neutralization and control experiments.
Target Information
FOXA3 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fork head-related protein FKH H3; Forkhead box protein A3; hepatocyte nuclear factor 3 gamma (winged helix transcription factor); hepatocyte nuclear factor 3, gamma; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; TCF-3G; Transcription factor 3G
Gene Aliases: FKHH3; FOXA3; Hnf-3g; HNF3G; Tcf-3g; TCF3G
UniProt ID: (Human) P55318, (Mouse) P35584, (Rat) P32183
Entrez Gene ID: (Human) 3171, (Mouse) 15377, (Rat) 25100
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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