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Application
FIGURE: 1 / 3
PAG1 Antibody (MA5-57149) in ICC/IF
Immunocytochemical staining of PAG1 in HT-1080 cells using PAG1 Recombinant Monoclonal Antibody (Product # MA5-57149) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; PAG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Product Details
MA5-57149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB685
Immunogen
A synthesized peptide derived from human PAG1 (aa 400-432)
Conjugate
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_3680174
Target Information
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Csk binding protein; Csk-binding protein; phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein transmembrane adaptor 1; phosphoprotein-associated with GEMs; Transmembrane adapter protein PAG; transmembrane adaptor protein PAG; Transmembrane phosphoprotein Cbp
Gene Aliases: CBP; F730007C19Rik; PAG; PAG1
UniProt ID: (Human) Q9NWQ8, (Rat) Q9JM80, (Mouse) Q3U1F9
Entrez Gene ID: (Human) 55824, (Rat) 64019, (Mouse) 94212
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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