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Invitrogen
PC4 Monoclonal Antibody (04)
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Application
FIGURE: 1 / 3
PC4 Antibody (MA5-29588) in ICC/IF
Immunofluorescence staining of Human PC4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PC4 Monoclonal Antibody (4) (Product # MA5-29588, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.
Product Details
MA5-29588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
04
Immunogen
Recombinant Human SUB1/PC4 protein (Met1-Leu127)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785429
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SUB1/PC4.
Target Information
PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activated RNA polymerase II transcription cofactor 4; Activated RNA polymerase II transcriptional coactivator p15; p14; PC4; Positive cofactor 4; SUB1 homolog; TIS7
Gene Aliases: p14; P15; PC4; RPO2TC1; SUB1
UniProt ID: (Human) P53999
Entrez Gene ID: (Human) 10923
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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