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            • Primary Antibodies ›
            • PCAF Antibodies

            Bethyl Laboratories

            PCAF Polyclonal Antibody

            View all (31) PCAF antibodies
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            Datasheet
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            Cite PCAF Polyclonal Antibody

            • Antibody Testing Data (3)
            Application
            PCAF Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            PCAF Antibody (A301-666A) in WB

            Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody (Product # A301-666A) (lot A301-666A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
            View Product

            PCAF Antibody in Western Blot (WB)
            PCAF Antibody in Immunoprecipitation (IP)
            PCAF Antibody in ChIP-Sequencing (ChIP-seq)

            Product Details

            A301-666A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-10 µg/mg lysate
            -

            ChIP-sequencing (ChIP-Seq)

            2 µg
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 782 and 832 of human p300/CBP-associated factor.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            tris citrate/phosphate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Based on 100% sequence identity, this antibody is predicted to react with Mouse

            Target Information

            PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B

            View more View less

            Gene Aliases: CAF; KAT2B; P/CAF; PCAF

            View more View less

            UniProt ID: (Human) Q92831

            View more View less

            Entrez Gene ID: (Human) 8850

            View more View less

            Function(s)

            RNA polymerase II regulatory region sequence-specific DNA binding chromatin binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding transcription factor binding acetyltransferase activity protein kinase binding protein complex binding histone acetyltransferase binding histone deacetylase binding

            Process(es)

            chromatin remodeling transcription initiation from RNA polymerase II promoter protein acetylation cell cycle arrest Notch signaling pathway memory negative regulation of cell proliferation regulation of protein ADP-ribosylation positive regulation of neuron projection development viral process N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation peptidyl-lysine acetylation cellular response to insulin stimulus cellular response to oxidative stress positive regulation of chromatin binding positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter histone H3 acetylation histone H3-K9 acetylation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of gene expression, epigenetic positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter rhythmic process cellular response to parathyroid hormone stimulus positive regulation of histone H3-K14 acetylation positive regulation of histone H3-K9 acetylation
            It has to be done as per old AB suggested Products section.
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