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PCIF1 Polyclonal Antibody

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Antibody Testing Data (11)

PCIF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

PCIF1 Antibody (16082-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using (Product # 16082-1-AP) (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

16082-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCIF1 Fusion Protein Ag9080 (355-704 aa encoded by BC013365)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DSVEGICSK IYHISLEYVK RIREKHLAIL KENNISEEVE APEVEPRLVY CYPVRLAVSA PPMPSVEMHM ENNVVCIRYK GEMVKVSRNY FSKLWLLYRY SCIDDSAFER FLPRVWCLLR RYQMMFGVGL YEGTGLQGSL PVHVFEALHR LFGVSFECFA SPLNCYFRQY CSAFPDTDGY FGSRGPCLDF APLSGSFEAN PPFCEELMDA MVSHFERLLE SSPEPLSFIV FIPEWREPPT PALTRMEQSR FKRHQLILPA FEHEYRSGSQ HICKKEEMHY KAVHNTAVLF LQNDPGFAKW APTPERLQEL SAAYRQSGRS HSSGSSSSSS SEAKDRDSGR EQGPSREPHP T (355-704 aa encoded by BC013365)

Target Information

PCIF1 may play a role in transcription elongation or in coupling transcription to pre-mRNA processing through its association with the phosphorylated C-terminal domain (CTD) of RNAPII largest subunit.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PCIF1-mediated deposition of 5'-cap N⁶,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection.

Proceedings of the National Academy of Sciences of the United States of America

Wang L,Wang S,Wu L,Li W,Bray W,Clark AE,Gonzalez GM,Wang Y,Carlin AF,Rana TM

Tue Jan 31 00:00:00 EST 2023

Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.

The EMBO journal

Wang L,Wu L,Zhu Z,Zhang Q,Li W,Gonzalez GM,Wang Y,Rana TM

Mon Jan 16 00:00:00 EST 2023

Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.

The EMBO journal

Wang L,Wu L,Zhu Z,Zhang Q,Li W,Gonzalez GM,Wang Y,Rana TM

Mon Jan 16 00:00:00 EST 2023

Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.

The EMBO journal

Wang L,Wu L,Zhu Z,Zhang Q,Li W,Gonzalez GM,Wang Y,Rana TM

Mon Jan 16 00:00:00 EST 2023

Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.

The EMBO journal

Wang L,Wu L,Zhu Z,Zhang Q,Li W,Gonzalez GM,Wang Y,Rana TM

Mon Jan 16 00:00:00 EST 2023

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Molecular cell

Li Y,Huang J,Bao L,Zhu J,Duan W,Zheng H,Wang H,Jiang Y,Liu W,Zhang M,Yu Y,Yi C,Ji X

Thu Apr 20 00:00:00 EDT 2023

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Molecular cell

Li Y,Huang J,Bao L,Zhu J,Duan W,Zheng H,Wang H,Jiang Y,Liu W,Zhang M,Yu Y,Yi C,Ji X

Thu Apr 20 00:00:00 EDT 2023

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Molecular cell

Li Y,Huang J,Bao L,Zhu J,Duan W,Zheng H,Wang H,Jiang Y,Liu W,Zhang M,Yu Y,Yi C,Ji X

Thu Apr 20 00:00:00 EDT 2023

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Molecular cell

Li Y,Huang J,Bao L,Zhu J,Duan W,Zheng H,Wang H,Jiang Y,Liu W,Zhang M,Yu Y,Yi C,Ji X

Thu Apr 20 00:00:00 EDT 2023

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.

Cell reports

Jansens RJJ,Verhamme R,Mirza AH,Olarerin-George A,Van Waesberghe C,Jaffrey SR,Favoreel HW

Tue Jul 19 00:00:00 EDT 2022

HIV reprograms host m⁶Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1.

Nature communications

Zhang Q,Kang Y,Wang S,Gonzalez GM,Li W,Hui H,Wang Y,Rana TM

Mon Sep 20 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Cap-specific adenosine methyltransferase; CAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PDX-1 C terminus-interacting factor 1; phosphorylated CTD interacting factor PCIF1; Phosphorylated CTD-interacting factor 1; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121

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Gene Aliases: 2310022K11Rik; C20orf67; CAPAM; F730014I05Rik; PCIF1; PPP1R121

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UniProt ID: (Human) Q9H4Z3, (Mouse) P59114

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Entrez Gene ID: (Human) 63935, (Mouse) 228866

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Function(s)

molecular_function

Process(es)

negative regulation of phosphatase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PCIF1 Polyclonal Antibody

PCIF1 Antibody (16082-1-AP) in IHC (P)

Product Details

16082-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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