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Invitrogen
PCM1 Polyclonal Antibody
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Application
FIGURE: 1 / 10
PCM1 Antibody (PA5-54779) in ICC/IF
Immunofluorescence analysis of PCM1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PCM1 Polyclonal Antibody (Product # PA5-54779) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More
Product Details
PA5-54779
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human PCM1. Recombinant protein control fragment (Product #RP-93739).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.30 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2645287
Product Specific Information
Immunogen sequence: TIYSEVATLI SQNESRPHFL IELFHELQLL NTDYLRQRAL YALQDIVSRH ISESHEKGEN VKSVNSGTWI ASNSELTPSE SLATTDDETF EKNFE
Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 95%.
Target Information
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPCM-1; PCM-1; Pericentriolar material 1 protein; pericentriolar material 1, PCM1
Gene Aliases: PCM1; PTC4; RET/PCM-1
UniProt ID: (Human) Q15154
Entrez Gene ID: (Human) 5108
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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