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PCMT1 Antibody (MA5-27236) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a PCMT1 monoclonal antibody (Product # MA5-27236) at a dilution of 1:2000.
Product Details
MA5-27236
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI2A9
Immunogen
Human recombinant protein fragment corresponding to amino acids 60-285 of PCMT1 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2724959
Target Information
Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L-isoaspartyl protein carboxyl methyltransferase; PIMT; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase; Protein-L-isoaspartate(D-aspartate) O-methyltransferase
Gene Aliases: PCMT1; PIMT
UniProt ID: (Human) P22061
Entrez Gene ID: (Human) 5110
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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