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PDH Antibody (45-6799) in ICC/IF
Immunofluorescence analysis of PDH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PDH Antibody Cocktail (Product # 45-6799) at 1:250 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11029, 1:2,000 dilution) for 4... View More
Product Details
45-6799
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ Ig
Class
Cocktail
Type
Antibody
Clone
Cocktail
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
IgG fraction
Storage buffer
HEPES buffered saline, pH 7.5
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_2533826
Product Specific Information
Anti-PDH Western Blot Kit; Western Blot kit (Cocktail of monoclonal antibodies) specific to Human, bovine PDH. This antibody is validated for use in Western Blot.
C-V-OSCP (clone ) 4C11C10D12 - Antigen Human rec. mature OSCP
Amount: 12.5 µg , Working concentration: 0.5 µg/mL
PDH-E1-alpha (clone) 9H9AF5 - Human rec. PDH-E1-a
Amount: 25 µg , Working concentration: 1 µg/mL
PDH-E1-beta (clone) 17A5E2H8 - Porcine PDH
Amount: 62.5 µg, Working concentration: 2.5 µg/mL
PDH-E2 (clone) 15D3G9C11 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL
PDH-E2/E3bp (clone) 13G2AE2BH5 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL
Target Information
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 13G2AE2BH5; 15D3G9C11; 17A5E2H8; 4C11C10D12; 9H9AF5; PDHE1-A type I; PDHE1-A type II; PDHE1-B; pyruvate dehydrogenase; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase (lipoamide) alpha 2; pyruvate dehydrogenase (lipoamide) beta; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase alpha 2; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 alpha 2; pyruvate dehydrogenase E1 alpha 2 subunit; pyruvate dehydrogenase E1 beta subunit; pyruvate dehydrogenase E1 component alpha subunit; PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A TYPE II); Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit; pyruvate dehydrogenase, E1 beta polypeptide; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; RP11-723P2.1
Gene Aliases: 2610103L06Rik; AL024199; C81408; PDHA; Pdha-1; Pdha-2; PDHA1; PDHA2; PDHAD; PDHAL; PDHB; PDHBD; PDHCE1A; PDHE1-B; PHE1A; PHE1B
pyruvate dehydrogenase activity
pyruvate dehydrogenase (acetyl-transferring) activity
pyruvate dehydrogenase (NAD+) activity
protein binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
catalytic activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
glucose metabolic process
acetyl-CoA biosynthetic process from pyruvate
pyruvate metabolic process
tricarboxylic acid cycle
regulation of acetyl-CoA biosynthetic process from pyruvate
glyoxylate metabolic process
mitochondrial acetyl-CoA biosynthetic process from pyruvate
glycolytic process
oxidation-reduction process
carbohydrate metabolic process
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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