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Invitrogen

PDK1 Recombinant Rabbit Monoclonal Antibody (5T6S8)

View all (18) PDK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

PDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

PDK1 Antibody (MA5-35736) in IHC (P)

Immunohistochemistry analysis of PDK1 in paraffin-embedded rat kidney. Samples were incubated with PDK1 Monoclonal antibody (Product # MA5-35736) using a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Product Details

MA5-35736

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:5,000

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5T6S8

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 337-436 of human PDK1/PDHK1 (NP_0026011)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2849636

Product Specific Information

Immunogen sequence: STAPRPRVET SRAVPLAGFG YGLPISRLYA QYFQGDLKLY SLEGYGTDAV IYIKALSTDS IERLPVYNKA AWKHYNTNHE ADDWCVPSRE PKDMTTFRSA

Target Information

PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; PDH kinase 1; PDK p48; PkB kinase like gene 1; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1

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Gene Aliases: B830012B01; D530020C15Rik; PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

UniProt ID: (Human) O15530, (Mouse) Q8BFP9

Entrez Gene ID: (Human) 5170, (Rat) 116551, (Mouse) 228026

Function(s)

protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity insulin receptor binding protein binding ATP binding phospholipase activator activity kinase activity protein kinase binding phospholipase binding protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity protein complex binding protein homodimerization activity protein heterodimerization activity nucleotide binding transferase activity non-receptor serine/threonine protein kinase

Process(es)

stimulatory C-type lectin receptor signaling pathway type B pancreatic cell development transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity hyperosmotic response epidermal growth factor receptor signaling pathway positive regulation of phospholipase activity regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling actin cytoskeleton organization platelet activation negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation activation of protein kinase B activity cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction Fc-epsilon receptor signaling pathway regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein autophosphorylation phosphatidylinositol-mediated signaling focal adhesion assembly T cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway glucose metabolic process cell surface receptor signaling pathway cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress regulation of acetyl-CoA biosynthetic process from pyruvate regulation of glucose metabolic process hypoxia-inducible factor-1alpha signaling pathway carbohydrate metabolic process phosphorylation mitophagy in response to mitochondrial depolarization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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