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NeoBiotechnologies
PDZ and LIM Domain 1 Monoclonal Antibody (CPTC-PDLIM1-1)
Application
FIGURE: 1 / 2
PDZ and LIM Domain 1 Antibody (9124-MSM1-P1) in IHC (P)
Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human Breast carcinoma using PDZ and LIM Domain 1 Monoclonal Antibody (Product # 9124-MSM1-P1).
Product Details
9124-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
CPTC-PDLIM1-1
Immunogen
Recombinant human full-length PDLIM1 protein
Conjugate
Unconjugated
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-terminal LIM domain protein 1; carboxyl terminal LIM domain protein 1; Elfin; enigma; epididymis secretory protein Li 112; LIM domain protein CLP-36; PDZ and LIM domain protein 1
Gene Aliases: CLIM1; CLP-36; CLP36; hCLIM1; HEL-S-112; PDLIM1
UniProt ID: (Human) O00151
Entrez Gene ID: (Human) 9124
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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