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Bethyl Laboratories
PELO Polyclonal Antibody
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Application
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PELO Antibody (A305-447A) in IP
Detection of human PELO by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PELO antibody (Product # A305-447A) (lot 1) used for IP at 6 µg per reaction. PELO was also immunoprecipitated by rabbit anti-PELO antibody BL21614. For blotting immunoprecipitated PELO, A305-447A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-447A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 to 50 of human Protein pelota homolog.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
PELO encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPelota; Protein Dom34 homolog; Protein pelota homolog
Gene Aliases: CGI-17; PELO; PRO1770
UniProt ID: (Human) Q9BRX2
Entrez Gene ID: (Human) 53918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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