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Application
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PER1 Antibody (MA5-58132) in IHC (P)
Immunohistochemistry analysis of PER1 with paraffin-embedded Human colon carcinoma tissue. The tissue was incubated with PER1 Recombinant Monoclonal Antibody (Product # MA5-58132) using a 1:300 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.
Product Details
MA5-58132
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2Y1M7
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.28 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MSGPLEGADG GGDPRPGESF CPGGVPSPGP PQHRPCPGPS LADDTDANSN GSSGNESNGH ESRGASQRSS HSSSSGNGKD SALLETTESS KSTNSQSPSP PSSSIAYSLL SASSEQDNPS TSGCSSEQSA RARTQKELMT ALRELKLRLP PERRGKGRSG TLATLQYALA CVKQVQANQE YYQQWSLEEG EPCSMDMSTY
Target Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; mPER1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of
Gene Aliases: hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI
UniProt ID: (Human) O15534, (Mouse) O35973
Entrez Gene ID: (Human) 5187, (Mouse) 18626
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcription factor binding
kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
E-box binding
transcription regulatory region sequence-specific DNA binding
signal transducer activity
protein binding
transcription cofactor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
regulation of sodium ion transport
transcription, DNA-templated
circadian rhythm
entrainment of circadian clock
posttranscriptional regulation of gene expression
circadian regulation of gene expression
regulation of hair cycle
regulation of circadian rhythm
negative regulation of I-kappaB kinase/NF-kappaB signaling
entrainment of circadian clock by photoperiod
histone H3 acetylation
histone H4 acetylation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
response to cAMP
histone H3 deacetylation
circadian regulation of translation
regulation of cytokine production involved in inflammatory response
regulation of p38MAPK cascade
negative regulation of glucocorticoid receptor signaling pathway
regulation of transcription, DNA-templated
signal transduction
rhythmic process
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