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Application
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PERK Antibody (PA5-79193) in WB
Western blot was performed using anti-PERK Polyclonal Antibody (Product # PA5-79193) and 125 kDa band corresponding to PERK was observed across the cell lines tested, also, the protein expression was increased upon Thapsigargin treatment in MCF7 and U-87 MG along with uncharacterized bands (*). Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with Thapsigargin (1 µMol/L for 24 hrs) (Lane 2), U-87 MG (Lane 3), U-87 MG treated with Thapsigargin (1 µMol/L for 24 hrs) (Lane 4), HeLa (Lane 5), T-47D (Lane 6), Hep G2 (Lane 7) an... View More
Product Details
PA5-79193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human PERK recombinant protein (Position: R222-Q334).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746309
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human COLO-320 whole cell, human A549 whole cell, human SK-OV-3 whole cell, Human A431 whole cell, rat brain tissue, mouse brain tissue. Flow: HepG2 cell.
Target Information
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3
Gene Aliases: AI427929; EIF2AK3; PEK; PERK; WRS
UniProt ID: (Human) Q9NZJ5, (Rat) Q9Z1Z1, (Mouse) Q9Z2B5
Entrez Gene ID: (Human) 9451, (Rat) 29702, (Mouse) 13666
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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