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            • Primary Antibodies ›
            • PFKM Antibodies

            Bethyl Laboratories

            PFKM Polyclonal Antibody

            View all (25) PFKM antibodies

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            Catalog # A304-256A
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            Cite PFKM Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            PFKM Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            PFKM Antibody (A304-256A) in WB

            Detection of human and mouse PFKM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PFKM antibody (Product # A304-256A) (lot A304-256A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
            View Product

            PFKM Antibody in Western Blot (WB)
            PFKM Antibody in Immunoprecipitation (IP)

            Product Details

            A304-256A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-10 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 730 to 780 of human Phosphofructokinase, Muscle.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            phosphate/tris citrate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan

            Target Information

            Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: 6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-A; Phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase 4; phosphofructokinase M variant 3/4; phosphofructokinase muscle; phosphofructokinase, polypeptide X; phosphofructokinase-1 A isozyme; phosphofructokinase-M; phosphofructokinase-M (connecting peptide); Phosphohexokinase; protein phosphatase 1, regulatory subunit 122

            View more View less

            Gene Aliases: AI131669; ATP-PFK; GSD7; PFK-1; Pfk-4; PFK-A; PFK-M; PFK1; Pfk4; PFKA; PFKM; PFKX; PPP1R122

            View more View less

            UniProt ID: (Human) P08237, (Mouse) P47857

            View more View less

            Entrez Gene ID: (Human) 5213, (Mouse) 18642

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            Function(s)

            6-phosphofructokinase activity protein binding ATP binding protein C-terminus binding kinase binding identical protein binding protein homodimerization activity metal ion binding fructose binding nucleotide binding catalytic activity phosphofructokinase activity AMP binding kinase activity transferase activity carbohydrate binding fructose-6-phosphate binding carbohydrate kinase kinase transferase metabolite interconversion enzyme

            Process(es)

            glycogen catabolic process fructose 6-phosphate metabolic process glycolytic process positive regulation of insulin secretion glucose homeostasis muscle cell cellular homeostasis carbohydrate phosphorylation protein oligomerization canonical glycolysis glycolysis from storage polysaccharide through glucose-1-phosphate metabolic process phosphorylation fructose 1,6-bisphosphate metabolic process protein homotetramerization glycolytic process through fructose-6-phosphate
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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