Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
PHD1 Recombinant Rabbit Monoclonal Antibody (3G4)
Promotions
View available promotion(s)
Promo Code: P5906365 Don't miss out: buy 3, only pay for 2 Extended to June 27: take advantage of our 3 for 2 promotion. Whether you are restocking essentials or trying something new, now is a great time to get more for your money!. Learn more
Promo Code: RPUZZ25 Stock up on essentials to piece your discovery together Until June 27, save up to $650 and get an exclusive lab-themed hidden-object puzzle. Learn more
Application
FIGURE: 1 / 7
PHD1 Antibody (MA5-24853) in ICC/IF
Immunofluorescence analysis of PHD1 was performed using 70% confluent log phase HeLa cells treated with Cobalt chloride. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHD1 Recombinant Rabbit Monoclonal Antibody (3G4) (Product # MA5-24853) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant... View More
Product Details
MA5-24853
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3G4
Immunogen
Synthetic peptide of human hydroxylase PHD1 (EGLN2) (aa 21-34).
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717291
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Purity is > 95% by SDS-PAGE.
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13. 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Egl nine homolog 2; egl-9 family hypoxia-inducible factor 2; EIT-6; Estrogen-induced tag 6; Falkor; HIF-1alpha prolyl-4-hydroxylase-1; HIF-P4H 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; immediate early response 4; P4H1; PHD1; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase EGLN2
Gene Aliases: 0610011A13Rik; C85656; EGLN2; EIT6; Hif-p4h-1; HIF-PH1; HIFPH1; HPH-1; HPH-3; Ier4; PHD-1; PHD1; SM-20
UniProt ID: (Human) Q8WWY4, (Mouse) Q91YE2, (Rat) Q6AYU4
Entrez Gene ID: (Human) 112398, (Mouse) 112406, (Rat) 308457
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support