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PHGDH Antibody (PA5-27578) in IHC (P)
PHGDH Polyclonal Antibody detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. PHGDH Polyclonal Antibody (Product # PA5-27578) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-27578
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 175 of Human PHGDH
Conjugate
Form
Liquid
Concentration
0.87 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545054
Product Specific Information
Recommended positive controls: HeLa, 293T, A431, HeLaS3, mouse brain, SK-N-DZ.^M
^M
Predicted reactivity: Mouse (99%), Rat (97%), Pig (97%), Chimpanzee (100%), Bovine (97%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3-phosphoglycerate dehyrogenase; A10; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase
Gene Aliases: 3-PGDH; 3PGDH; 4930479N23; A10; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA
UniProt ID: (Human) O43175, (Mouse) Q61753, (Rat) O08651
Entrez Gene ID: (Human) 26227, (Mouse) 236539, (Rat) 58835
glutamine metabolic process
glycine metabolic process
L-serine biosynthetic process
threonine metabolic process
brain development
serine family amino acid biosynthetic process
gamma-aminobutyric acid metabolic process
regulation of gene expression
taurine metabolic process
spinal cord development
glial cell development
neural tube development
neuron projection development
oxidation-reduction process
G1 to G0 transition
L-serine metabolic process
metabolic process
cellular amino acid biosynthetic process
neurogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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