Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
PIEZO1 Polyclonal Antibody
Application
FIGURE: 1 / 10
PIEZO1 Antibody (PA5-106296) in ICC/IF
Immunofluorescence analysis of Piezo1 was performed using 70% confluent log phase U-87 MG subjected to a mechanical stress of ~2g/cm2 for 30 mins. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PIEZO1 Polyclonal Antibody (Product # PA5-106296) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Ant... View More
Product Details
PA5-106296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIEZO1(Accession Q92508), corresponding to amino acid residues I642-L692.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853973
Product Specific Information
Antibody detects endogenous levels of total Piezo1.
Target Information
Piezo1 is a pore-forming subunit of a mechanosensitive non-specific cation channel, and generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Structurally, Piezo1 contains 36 transmembrane domains and functions as a homotetramer. Piezo1 plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, Piezo1 may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Piezo1 acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation and plays a key role in blood vessel formation and vascular structure in both development and adult physiology. The protein encoded by the Piezo1 gene is a mechanically-activated ion channel that links mechanical forces to biological signals. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. Diseases associated with PIEZO1 include Dehydrated Hereditary Stomatocytosis with/without Pseudohyperkalemia And/Or Perinatal Edema and Lymphedema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: family with sequence similarity 38, member A; Membrane protein induced by beta-amyloid treatment; Mib; Piezo-type mechanosensitive ion channel component 1; Protein FAM38A; protein PIEZO1
Gene Aliases: 9630020g22; DHS; FAM38A; KIAA0233; LMPH3; Mib; mKIAA0233; PIEZO1
UniProt ID: (Human) Q92508, (Rat) Q0KL00
Entrez Gene ID: (Human) 9780, (Rat) 361430, (Mouse) 234839
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support