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            • Primary Antibodies ›
            • PIGY Antibodies

            Invitrogen

            PIGY Polyclonal Antibody

            View all (3) PIGY antibodies

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            Datasheet
            Protocols
            Questions & Answers
            Catalog # PA5-20651
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            Price (USD)
            487.00
            100 µg
             
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            Cite PIGY Polyclonal Antibody

            • Antibody Testing Data (3)
            Application
            PIGY Antibody in Immunohistochemistry (IHC)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            PIGY Antibody (PA5-20651) in IHC

            Immunohistochemistry of PIGY in human spleen tissue with PIGY Polyclonal Antibody (Product # PA5-20651) at 2.5 µg/mL.
            View Product

            PIGY Antibody in Immunohistochemistry (IHC)
            PIGY Antibody in Immunohistochemistry (IHC)
            PIGY Antibody in Western Blot (WB)

            Product Details

            PA5-20651

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            5 µg/mL
            -

            Immunohistochemistry (IHC)

            2.5 µg/mL
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A 16 amino acid peptide near the carboxy terminus of human PIG-Y.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS

            Contains

            0.02% sodium azide

            Storage conditions

            Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_11153820

            Product Specific Information

            Despite its predicted molecular weight, PIG-Y often migrates at 28-30kDa in SDS-PAGE. A suggested positive control is A-20 cell lysate.

            PA5-20651 can be used with blocking peptide PEP-0771.

            Target Information

            Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Let us know so we can reference it here.
            Cite this product

            Bioinformatics

            Protein Aliases: Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-Y; PREY; protein preY

            View more View less

            Gene Aliases: 1810008A14Rik; HPMRS6; PIG-Y; PIGY; Pigyl

            View more View less

            UniProt ID: (Human) Q3MUY2, (Mouse) P0C1P0

            View more View less

            Entrez Gene ID: (Human) 84992, (Mouse) 66268

            View more View less

            Function(s)

            molecular_function transferase

            Process(es)

            GPI anchor biosynthetic process biological_process
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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