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Despite its predicted molecular weight, PIG-Y often migrates at 28-30kDa in SDS-PAGE. A suggested positive control is human spleen tissue lysate.
PA5-20652 can be used with blocking peptide PEP-0772.
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-Y; PREY; protein preY
Gene Aliases: 1810008A14Rik; HPMRS6; PIG-Y; PIGY; Pigyl
UniProt ID: (Human) Q3MUY2, (Mouse) P0C1P0
Entrez Gene ID: (Human) 84992, (Mouse) 66268
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