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Invitrogen

PIT1 Polyclonal Antibody

1 Reference

View all (5) PIT1 antibodies

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Catalog # PA5-59662

Price (KRW)

682,000

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Ends: 30-Jun-2025

802,000

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100 µL

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)

Application

PIT1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 6

PIT1 Antibody (PA5-59662) in IHC

Immunohistochemical staining of PIT1 in human pituitary gland using PIT1 Polyclonal Antibody (Product # PA5-59662) shows moderate to strong nuclear positivity in neuroendocrine cells in the anterior lobe.

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Product Details

PA5-59662

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PIT1. Recombinant protein control fragment (Product #RP-98326).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2645576

Product Specific Information

Immunogen sequence: CKLKAILSKW LEEAEQVGAL YNEKVGANER KRKRRTTISI AAKDALERHF GEQNKPSSQE IMRMAE

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 97%.

Target Information

PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?

Cancers

Torregrosa-Quesada ME,García-Martínez A,Silva-Ortega S,Martínez-López S,Cámara R,Fajardo C,Lamas C,Aranda I,Picó A

PA5-59662 was used in Immunohistochemistry to quantify quantification of pituitary-specific TF gene expression by RT-qPCR to overcome the shortcomings of IHC and to complement it.

Wed Dec 11 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: GHF-1; Growth hormone factor 1; PIT-1; Pituitary-specific positive transcription factor 1; POU domain, class 1, transcription factor 1

Gene Aliases: CPHD1; GHF-1; GHF1; Pit-1; PIT1; POU1F1; POU1F1a

UniProt ID: (Human) P28069

Entrez Gene ID: (Human) 5449

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter cell fate specification regulation of transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of cell proliferation determination of adult lifespan B cell differentiation positive regulation of inositol trisphosphate biosynthetic process positive regulation of multicellular organism growth regulation of insulin-like growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter nuclear transport somatotropin secreting cell development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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