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            • Primary Antibodies ›
            • PITX1 Antibodies

            Bethyl Laboratories

            PITX1 Polyclonal Antibody

            View all (11) PITX1 antibodies

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            Datasheet
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            Catalog # A300-577A
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            Cite PITX1 Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            PITX1 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            PITX1 Antibody (A300-577A) in WB

            Detection of human PITX by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody (Product # A300-577A) (lot A300-577A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
            View Product

            PITX1 Antibody in Western Blot (WB)
            PITX1 Antibody in Immunoprecipitation (IP)

            Product Details

            A300-577A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-10 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residues 50 and 100 of Human Paired-Like Homeodomain Transcription Factor 1.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            tris citrate/phosphate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Target Information

            PITX1 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: hindlimb expressed homeobox protein backfoot; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; OTX; paired-like homeodomain 1; Paired-like homeodomain transcription factor 1; pituitary homeo box 1; Pituitary homeobox 1; pituitary otx-related factor

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            Gene Aliases: BFT; CCF; LBNBG; PITX1; POTX; PTX1

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            UniProt ID: (Human) P78337

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            Entrez Gene ID: (Human) 5307

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            Function(s)

            RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding protein binding homeodomain transcription factor

            Process(es)

            skeletal system development transcription from RNA polymerase II promoter anatomical structure morphogenesis branchiomeric skeletal muscle development pituitary gland development embryonic hindlimb morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter myoblast fate commitment cartilage development
            It has to be done as per old AB suggested Products section.
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