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Proteintech

PJA1 Polyclonal Antibody

8 References

View all (6) PJA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Application

FIGURE: 1 / 4

PJA1 Antibody (17687-1-AP) in WB

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PJA1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17687-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PJA1 Fusion Protein Ag11820 (294-643 aa encoded by BC075803)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ANDNEGHSD GLARRGRGES SSGYPEPKYP EDKREARSDQ VKPEKVPRRR RTMADPDFWT HSDDYYKYCD EDSDSDKEWI AALRRKYRSR EQTLSSSGES WETLPGKEER EPPQAKVSAS TGTSPGPGAS ASAGAGAGAS AGSNGSNYLE EVREPSLQEE QASLEEGEIP WLQYHENDSS SEGDNDSGHE LMQPGVFMLD GNNNLEDDSS VSEDLEVDWS LFDGFADGLG VAEAISYVDP QFLTYMALEE RLAQAMETAL AHLESLAVDV EVANPPASKE SIDALPEILV TEDHGAVGQE MCCPICCSEY VKGEVATELP CHHYFHKPCV SIWLQKSGTC PVCRCMFPPP L (294-643 aa encoded by BC075803)

Target Information

Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

OTUB2 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells by PJA1 Deubiquitylation.

Cellular and molecular bioengineering

Hu G,Yang J,Zhang H,Huang Z,Yang H

Wed Jun 01 00:00:00 UTC 2022

Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.

Neuropathology : official journal of the Japanese Society of Neuropathology

Watabe K,Niida-Kawaguchi M,Tada M,Kato Y,Murata M,Tanji K,Wakabayashi K,Yamada M,Kakita A,Shibata N

Thu Dec 01 00:00:00 UTC 2022

A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders.

Annals of clinical and translational neurology

Suzuki T,Suzuki T,Raveau M,Miyake N,Sudo G,Tsurusaki Y,Watanabe T,Sugaya Y,Tatsukawa T,Mazaki E,Shimohata A,Kushima I,Aleksic B,Shiino T,Toyota T,Iwayama Y,Nakaoka K,Ohmori I,Sasaki A,Watanabe K,Hirose S,Kaneko S,Inoue Y,Yoshikawa T,Ozaki N,Kano M,Shimoji T,Matsumoto N,Yamakawa K

Wed Jul 01 00:00:00 UTC 2020

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

Neuropathology : official journal of the Japanese Society of Neuropathology

Watabe K,Kato Y,Sakuma M,Murata M,Niida-Kawaguchi M,Takemura T,Hanagata N,Tada M,Kakita A,Shibata N

Tue Dec 01 00:00:00 UTC 2020

Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Lu Q,Murakami C,Hoshino F,Murakami Y,Sakane F

Sun Mar 01 00:00:00 UTC 2020

PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner.

Journal of virology

Xu W,Ma C,Zhang Q,Zhao R,Hu D,Zhang X,Chen J,Liu F,Wu K,Liu Y,Wu J

Thu Nov 15 00:00:00 UTC 2018

PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner.

Journal of virology

Xu W,Ma C,Zhang Q,Zhao R,Hu D,Zhang X,Chen J,Liu F,Wu K,Liu Y,Wu J

Thu Nov 15 00:00:00 UTC 2018

Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.

Nature communications

Consalvi S,Brancaccio A,Dall'Agnese A,Puri PL,Palacios D

Mon Jan 09 00:00:00 UTC 2017

Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.

Nature communications

Consalvi S,Brancaccio A,Dall'Agnese A,Puri PL,Palacios D

Mon Jan 09 00:00:00 UTC 2017

Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.

Nature communications

Consalvi S,Brancaccio A,Dall'Agnese A,Puri PL,Palacios D

Mon Jan 09 00:00:00 UTC 2017

Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.

Nature communications

Consalvi S,Brancaccio A,Dall'Agnese A,Puri PL,Palacios D

Mon Jan 09 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase Praja-1; praja 1; praja ring finger 1, E3 ubiquitin protein ligase; Praja1; praja1, RING-H2 motif containing; RING finger protein 70; RING-type E3 ubiquitin transferase Praja-1

Gene Aliases: PJA1; praja-1; PRAJA1; RNF70

UniProt ID: (Human) Q8NG27, (Mouse) O55176

Entrez Gene ID: (Human) 64219, (Rat) 683077, (Mouse) 18744

Function(s)

protein binding zinc ion binding ligase activity ubiquitin protein ligase activity ubiquitin-protein transferase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PJA1 Polyclonal Antibody

PJA1 Antibody (17687-1-AP) in WB

Product Details

17687-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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