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Invitrogen
PKLR Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Product Details
PA5-27305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 178 and 550 of Human Pyruvate Kinase
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544781
Product Specific Information
Recommended positive controls: Jurkat, Raji, K562, rat liver, PKLR-transfected 293T.
Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (80%), Dog (93%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: isozymes R/L; L-PK; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; Pyruvate kinase PKLR; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and red blood cell; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Gene Aliases: PK1; PKL; Pklg; PKLR; PKR; PKRL; RPK
UniProt ID: (Human) P30613, (Rat) P12928
Entrez Gene ID: (Human) 5313, (Rat) 24651
response to hypoxia
ATP biosynthetic process
response to nutrient
response to heat
response to glucose
response to lithium ion
cellular response to insulin stimulus
response to ATP
pyruvate biosynthetic process
response to cAMP
canonical glycolysis
carbohydrate metabolic process
glycolytic process
response to metal ion
response to organic cyclic compound
phosphorylation
response to other organism
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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