Loading ...

Invitrogen

PKM2 Polyclonal Antibody

Name: PKM2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA523034
Price: 540.0 USD

Advanced Verification

1 Published Figure

4 References

View all (72) PKM2 antibodies

Datasheet

Protocols

Questions & Answers

Catalog # PA5-23034

Price (USD)

540.00

100 µL

Estimated availability date: 07-Jul-2025

Check your price

Request bulk or custom format

Join Aspire^™ for free antibodies

Datasheet

Protocols

Questions & Answers

Application

PKM2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

PKM2 Antibody (PA5-23034) in ICC/IF

Immunofluorescence analysis of PKM2 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKM2 Polyclonal Antibody (Product # PA5-23034) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More

PKM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKM2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PKM2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MDAMB231 cells were transfected with PKM2 siRNA and reduction of signal was observed in Western Blot using PKM2 Polyclonal Antibody (Product # PA5-23034). {KD}

Product Details

PA5-23034

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

5 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11151984

Product Specific Information

The target sequence has 95% sequence homology with bovine.

Target Information

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TFEB inhibition induces melanoma shut-down by blocking the cell cycle and rewiring metabolism.

Cell death & disease

Ariano C,Costanza F,Akman M,Riganti C,Corà D,Casanova E,Astanina E,Comunanza V,Bussolino F,Doronzo G

PA5-23034 was used in Western Blot to suggest that the oncogenic transcription factor EB (TFEB), a key regulator of lysosomal biogenesis and function, controls melanoma tumour growth through a transcriptional programme targeting ERK1/2 activity and glucose, glutamine and cholesterol metabolism.

Tue May 09 00:00:00 UTC 2023

Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice.

Scientific reports

Delhove JM,Buckley SM,Perocheau DP,Karda R,Arbuthnot P,Henderson NC,Waddington SN,McKay TR

PA523034 was used in immunohistochemistry to report that hepatocytes rapidly upregulate NF- kB and Smad2/3 activity, and repress Notch signaling

Fri Feb 03 00:00:00 UTC 2017

p63 isoforms regulate metabolism of cancer stem cells.

Journal of proteome research

D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V

PA5-23034 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63

Fri Apr 04 00:00:00 UTC 2014

Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

Cell death & disease

Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A

PA5-23034 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells

Thu Jul 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2

Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1

UniProt ID: (Human) P14618, (Mouse) Q64484, (Rat) P11980

Entrez Gene ID: (Bovine) 512571, (Human) 5315, (Mouse) 18746, (Rat) 25630

Function(s)

magnesium ion binding pyruvate kinase activity protein binding ATP binding kinase activity MHC class II protein complex binding potassium ion binding ADP binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity transferase activity metal ion binding kinase

Process(es)

response to hypoxia liver development ATP biosynthetic process response to nutrient response to gravity programmed cell death response to muscle inactivity organ regeneration response to insulin pyruvate biosynthetic process skeletal muscle tissue regeneration canonical glycolysis cell-cell adhesion glucose metabolic process glycolytic process metabolic process phosphorylation response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PKM2 Polyclonal Antibody

PKM2 Antibody (PA5-23034) in ICC/IF

Product Details

PA5-23034

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!

PKM2 Polyclonal Antibody

PKM2 Antibody (PA5-23034) in ICC/IF

Product Details

PA5-23034

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Related Products for

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!