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PKN2 Antibody (PA5-22181) in IHC (P)
PRK2 antibody [N1N3] detects PRK2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PRK2 antibody [N1N3] (Product # PA5-22181) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-22181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 46 and 366 of PRK2 (Uniprot ID#Q16513)
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11155017
Product Specific Information
Recommended positive controls: A431, H1299, HeLa, HepG2, Raji.
Predicted reactivity: Mouse (94%), Rat (94%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PKN2 (PRK2), a close relative of the PKCs, is a regulator of mitosis and exit from cytokinesis. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiolipin-activated protein kinase Pak2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2; RP5-905H16.1; Serine/threonine-protein kinase N2
Gene Aliases: Pak-2; PAK2; PKN2; PRK2; PRKCL2; PRO2042; STK7
UniProt ID: (Human) Q9H1W4
Entrez Gene ID: (Human) 5586
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
apoptotic process
cell cycle
signal transduction
epithelial cell migration
peptidyl-serine phosphorylation
positive regulation of cytokinesis
intracellular signal transduction
apical junction assembly
positive regulation of viral genome replication
positive regulation of mitotic cell cycle
cell division
cell-cell adhesion
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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