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Invitrogen
PMCA1 ATPase Recombinant Rabbit Monoclonal Antibody (24GB5270)
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Application
FIGURE: 1 / 3
PMCA1 ATPase Antibody (MA5-56981) in ICC/IF
Immunocytochemical staining of PMCA1 ATPase in HepG2 cells using PMCA1 ATPase Recombinant Monoclonal Antibody (Product # MA5-56981) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-56981
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB5270
Immunogen
A synthesized peptide derived from human PMCA1 (aa 1100-1150)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.522
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_3680007
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 1; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 1; plasma membrane calcium pump; Plasma membrane calcium pump isoform 1; Plasma membrane calcium-transporting ATPase 1; PMCA1
Gene Aliases: 2810442I22Rik; ATP2B1; E130111D10Rik; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb
UniProt ID: (Human) P20020, (Mouse) G5E829, (Rat) P11505
Entrez Gene ID: (Human) 490, (Mouse) 67972, (Rat) 29598
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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