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Invitrogen
POLE2 Polyclonal Antibody
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Application
FIGURE: 1 / 2
POLE2 Antibody (PA5-103788) in IHC (P)
Immunohistochemistry analysis of POLE2 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with POLE2 polyclonal antibody (Product # PA5-103788) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103788
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POLE2(Accession P56282), corresponding to amino acid residues L477-F527.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853121
Product Specific Information
Antibody detects endogenous levels of total POLE2.
Target Information
DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol delta and epsilon. DNA pol delta consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol delta can be regulated by cell cycle proteins. DNA pol epsilon is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol delta and epsilon on leading and lagging strands have not yet been elucidated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA pol epsilon2; DNA pol epsilonB; DNA polymerase epsilon small subunit; DNA polymerase epsilon subunit 2; DNA polymerase epsilon subunit B; DNA polymerase epsilon, subunit 2; DNA polymerase II subunit 2; DNApol epsilon2; DNApol epsilonB; polymerase (DNA directed), epsilon 2 (p59 subunit); polymerase (DNA directed), epsilon 2, accessory subunit; polymerase (DNA) epsilon 2, accessory subunit
Gene Aliases: DPE2; POLE2
UniProt ID: (Human) P56282, (Mouse) O54956
Entrez Gene ID: (Human) 5427, (Mouse) 18974
Performance Guarantee
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