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Application
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POLG Antibody (PA5-29676) in IHC (P)
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DNA polymerase gamma (Product # PA5-29676) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29676
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 180 and 393 of Human DNA polymerase gamma
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547151
Product Specific Information
Recommended positive controls: NT2D1, rat testis.
Predicted reactivity: Mouse (82%), Rat (84%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; 5'-deoxyribose-phosphate lyase; DNA polymerase gamma; DNA polymerase subunit gamma-1; DNA-directed DNA polymerase gamma; FLJ27114; Mitochondrial DNA polymerase catalytic subunit; mitochondrial DNA polymerase gamma catalytic subunit; PolG-alpha; polymerase (DNA directed), gamma; polymerase (DNA) gamma, catalytic subunit; truncated mitochondrial DNA polymerase gamma catalytic subunit
Gene Aliases: MDP1; Mip1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG; POLG1; POLGA; SANDO; SCAE
UniProt ID: (Human) P54098, (Rat) Q9QYV8
Entrez Gene ID: (Human) 5428, (Rat) 85472
DNA metabolic process
DNA-dependent DNA replication
mitochondrial DNA replication
base-excision repair, gap-filling
aging
response to light stimulus
response to gamma radiation
response to hyperoxia
cellular response to glucose stimulus
DNA biosynthetic process
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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