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Invitrogen
PORCN Polyclonal Antibody
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Application
FIGURE: 1 / 1
PORCN Antibody (PA5-88445) in WB
Western blot analysis of PORCN in extracts of various cell lines. Samples were incubated with PORCN Polyclonal antibody (Product # PA5-88445) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88445
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_9822991)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804920
Product Specific Information
Immunogen sequence: PLNGDRLLRN KKRKARWLRA YESAVSFHFS NYFVGFLSEA TATLAGAGFT EEKDHLEWDL TVSKPLNVEL PRSMVEVVTS WNLPMSYWLN NYVFKNALRL G; Positive Samples: Mouse kidney, Mouse liver, Rat kidney; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
Target Information
PORCN belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC29687; mPORC; por; protein-cysteine N-palmitoyltransferase porcupine; Protein-serine O-palmitoleoyltransferase porcupine
Gene Aliases: 2410004O13Rik; AW045557; DXHXS7465e; Mg61; mMg61; Mporc; Porc; Porcn; Ppn; RGD1564947
UniProt ID: (Mouse) Q9JJJ7
Entrez Gene ID: (Mouse) 53627, (Rat) 317368
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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