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PPID Antibody (A305-335A) in IP
Detection of human PPID by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPID antibody A305-335A (lot A305-335A-1) used for IP at 6 µg per reaction. PPID was also immunoprecipitated by rabbit anti-PPID antibody A305-334A. For blotting immunoprecipitated PPID, A305-335A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-335A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 320 to 370 of Human Peptidyl-prolyl cis-trans isomerase D.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40 kDa peptidyl-prolyl cis-trans isomerase; 40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; cyclophilin D; Cyclophilin-40; Cyclophilin-related protein; Cyp 40; Cyp D; CYP-40; EC 5.2.1.8; MGC33096; Peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase; Rotamase D; testicular tissue protein Li 147
Gene Aliases: CYP-40; CYP40; CYPD; PPID
UniProt ID: (Human) Q08752
Entrez Gene ID: (Human) 5481
negative regulation of transcription from RNA polymerase II promoter
protein peptidyl-prolyl isomerization
protein folding
protein complex assembly
apoptotic process
protein transport
viral release from host cell
lipid particle organization
positive regulation of apoptotic process
positive regulation of viral genome replication
positive regulation of protein secretion
chaperone-mediated protein folding
cellular response to UV-A
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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