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PPIP5K2 Antibody (A304-167A) in WB
Detection of human PPIP5K2 by western blot. Samples: Whole cell lysate (10 µg) from K-562, GaMG, A-549, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (Product # A304-167A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-167A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 925 to 975 of Human Diphosphoinositol Pentakisphosphate Kinase 2.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
PPIP5K2, also known as inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinase 2, is a gene located on chromosome 5 in humans. This gene encodes a kinase involved in the phosphorylation of inositol pyrophosphates, which are essential for cellular signaling and regulation processes. Specifically, PPIP5K2 phosphorylates InsP6 and 1-InsP7 at the 5 position, playing a significant role in both cellular proliferation and metastasis. Recent studies have shown that PPIP5K2 can facilitate the growth and spread of non-small lung cancer (NSCLC) and colorectal carcinoma. It promotes cellular proliferation and migration independent of its kinase activity, in part by modifying its interaction with proteins like 14-3-3, affecting its subcellular distribution. Structural analysis of PPIP5K2 reveals that it possesses unique kinetic properties distinct from other inositol kinase isoforms. Genetic variants, such as the Arg837His missense variant, can alter its phosphatase activity, potentially impacting its functionality in signaling pathways. PPIP5K2 is intricately involved in epithelial-mesenchymal transition (EMT) mechanisms, further influencing cancer cell metastasis. Understanding the biochemical pathways and genetic variations associated with PPIP5K2 is crucial in comprehending its role in disease pathogenesis and therapeutic targeting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Diphosphoinositol pentakisphosphate kinase 2; histidine acid phosphatase domain containing 1; Histidine acid phosphatase domain-containing protein 1; hsVIP2; inositol heptaphosphate kinase 2; Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2; InsP6 and PP-IP5 kinase 2; mmVIP2; VIP1 homolog 2
Gene Aliases: AW555814; CFAP160; D330021B20; HISPPD1; IP7K2; KIAA0433; mKIAA0433; PPIP5K2; VIP2
UniProt ID: (Human) O43314, (Mouse) Q6ZQB6
Entrez Gene ID: (Human) 23262, (Mouse) 227399
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